[. . . ] [. . . ] Typical FCS3® Perfusion Configuration for Induced Change Experiments 1 Perfusion tubing, 1/16" ID supply line (keep as short as possible) 2 FCS3 Micro-Perfusion Pump (recommended for prolonged, controlled perfusion in delicate optical cavities) 3 Tee adapter (adds low volume syringe to facilitate manual perfusion when loading chamber) 4 Luer adapter (female, adapts Luer taper syringe to 1/16" tubing) 5 Low volume glass syringe 6 7 8 9 10 11 Luer adapter (male, adapts 1/16" tubing to female Luer lock) Alternate media glass syringe (growth factors, inhibitors, etc. ) Three-way valve (switches source of perfusion; pump or manual) FCS3 Chamber Tubing to waste container Tee adapter (place at same height as chamber, open top of tee to prevent siphoning) 3560 Beck Road · Butler, PA 16002 · Voice: 724-282-7145 · Fax: 724-282-0745 · E-mail: info@bioptechs. com · Web: www. bioptechs. com Note: Syringes are glass to prevent contamination of media from silicone lubricant typical in plastic syringes. [. . . ] [. . . ]